Optimizing Culture Conditions for in vitro Propagation of Trichosanthes cucumerina L.: An Important Medicinal Plant

摘要: ABSTRACT A simple and efficient, single medium–based protocol for rapid in vitro propagation was developed Trichosanthes cucumerina L., an important medicinal plant of the family Cucurbitaceae. The effects culture vessel, medium quantity per inoculation density were investigated, optimum conditions vigorous growth large-scale standardized. Axillary bud proliferation coupled with rooting from nodal explants obtained MS supplemented 0.46 μM kinetin 2.46 indole 3-butyric acid. highest response (97 %) multiplication rate 1:7 (seven shoots explant next subculture 4 weeks) achieved this medium. Inoculation five nodes bottle containing 30 ml optimum. Rooted plantlets could be established sand average 90% survival. micropropagated plants exhibited morphological similarity mother plants. results indica...