Association of CTLA-4, TNF alpha and IL 10 polymorphisms with susceptibility to hepatocellular carcinoma.

摘要: Our aim was to evaluate the association of genetic polymorphisms immunoregulatory molecules with susceptibility hepatocellular carcinoma (HCC). The in CTLA-4 (-318 T/C, CT60 G/A), TNF (-238 G/A, -308 G/A) and IL10 (-592 C/A, -819 C/T) were genotyped by PCR DNA sequencing. functional relevance examined ELISAs, vitro lymphocyte proliferation assay cytotoxic assay. -318 TC/TT, GG, -592 CA CT/TT variants, T IL 10 alleles positively associated HCC risk (P < .05). While -238 AA variant, A allele decreased Furthermore, combinations TC/TT GG/GA; CC; CC patients statistically significant Peripheral blood mononuclear cells (PBMCs) carrying genotypes exhibited significantly lower rates, IL-2, IL-4 levels, fewer cytolytic activities elevated TGF-β levels. For C/T, genotype higher rate, TGF-β, IL-10 levels only had serum In addition, we also found that T/C CT or TT, TC TT severity HCC. These findings suggest could promote pathogenesis HCC, which might be related down-regulation Th1/Th2-type cytokines and/or up-regulation Th3-type cytokines.