Fluorescent N-methylanthranilyl (Mantyl) tag for peptides: its application in subpicomole determination of kinins.
作者： Kalyan R. Anumula , Raymond P. Schulz , Nathan Back
DOI: 10.1016/0196-9781(92)90170-8
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摘要: Abstract Highly fluorescent N- methylanthranilyl (Mantyl) peptide derivatives were prepared by a one-step reaction with methylisatoic anhydride (MIA) for quantitative detection in HPLC. Reactions carried out an organic medium of acetonitrile-triethylamine, aqueous alkaline sodium carbonate and phosphate buffers. 4-Dimethylaminopyridine (DMAP) catalyzed specific mantylation -NH 2 groups peptides the medium. The DMAP had no effect buffered systems. Proline amino-terminal reacted equally well MIA. Mantyl-bradykinin excitation fluorescence maxima at 350 nm 426 water water/acetonitrile (ACN)/trifluoroacetic acid (TFA) solvent mixtures, respectively. Fluorescence intensity increased increase ACN concentration decreased content. Mantyl kinins completely resolved on C18 reversed phase HPLC column using ACN-0.1% TFA gradient their behavior was similar to having extra amino acid. Di-Mantyl obtained Lys-BK Met-Lys-BK did not exhibit appreciably higher than Mantyl-BK. about 50–100 times more sensitive (lower limits 0.1 0.5 picomole) UV phenylisothiocyanate-derivatized under typical conditions.
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