Determination of bradykinin in rat urine by coupled-column high pressure liquid chromatography with precolumn derivatization with a water-soluble fluorogenic reagent.
作者： Toshi Kajiro , Takeshi Fukushima , Kazuhiro Imai
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摘要: Abstract Bradykinin (BK) in rat urine was determined by coupled-column HPLC with precolumn fluorogenic derivatization a water-soluble reagent, 3-(7-fluoro-2,1,3-benzoxadiazole-4-sulfonamido)benzenesulfonic acid ( m -BS-ABD-F). The of BK -BS-ABD-F completed at 70°C for 100 min and gave only single peak derivative addition to the peaks blank. hydrophilicity reagent effectively prevented adsorption during sample pretreatment improved recovery BK. Good linearity shown between amount spiked (0–10 pmol) area derivatives (correlation coefficients >0.999), detection limits were 35 fmol (S/N = 3). precisions (cv, %) intra- interday assay not more than 5.5% accuracies range 95.3–111% (1 5 pmol urine, n Although regarded as that rapidly decreased after incubation 37°C, urinary kininase inhibitors samples drastically suppressed decrease this peak, confirming identified derivative. kinin excretion male SD rats (9–11 weeks old) present method 56.0 ± 22.1 pg/min/kg (mean SE, 5).
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