[Analytical chemical studies on high-performance recognition and detection of bio-molecules in life].
作者： Kazuhiro IMAI
关键词:
摘要: In order to understand the mechanism for maintaining life of animals based on search dynamics biomolecules, I have developed several sensitive and selective methods their quantification. Using derivatization with benzofurazan fluorogenic reagents (4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), ammonium 7-fluoro-2,1,3-benzoxadiazole 4-sulfonate (SBD-F) etc.) followed by high-performance liquid chromatography (HPLC)--fluorescence detection, a certain kind biological clinical importance was demonstrated chiral bio-molecules (D-amino acids, D-lactic acid so on), peptides proteins. The proposed method (derivatization SBD-F, isolation fluorescent proteins two-dimensional HPLC, enzymatic digestion identification altered HPLC-mass spectrometry (MS)/MS database-searching algorithm) proteomics studies revealed changed in islets Langerhans dexamethazone-induced diabetic rats. An catecholamine metabolism blood pressure regulation also suggested HPLC-chemiluminescence detection catecholamines 3-O-methylmetabolites. A new field Analytical Chemistry, i.e., Bio-Analytical proposed.
pharm.or.jp 参考文章(159)
Takeshi Fukushima, Sinya Adachi, Hideaki Ichihara, Salma Al-Kindy, Kazuhiro Imai, Fluorimetric determination of D- and L-lactate derivatized with 4-(N, N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) by high-performance liquid chromatography. Biomedical Chromatography. ,vol. 13, pp. 418- 424 ,(1999) , 10.1002/(SICI)1099-0801(199910)13:6<418::AID-BMC903>3.0.CO;2-E
Sakae Higashidate, Kazuhiro Imai, Pablo Prados, Satomi Adachi-Akahane, Taku Nagao, Relations between blood pressure and plasma norepinephrine concentrations after administration of diltiazem to rats: HPLC-peroxyoxalate chemiluminescence determination on an individual basis Biomedical Chromatography. ,vol. 8, pp. 19- 21 ,(1994) , 10.1002/BMC.1130080105
Natsuko Okiyama, Tomofumi Santa, Akira Toriba, Kazuhiro Imai, BF3–methanol as a cyclization/cleavage/conversion reagent for suppression of amino acid racemization in Edman sequencing and l-configuration determination method Analytica Chimica Acta. ,vol. 429, pp. 293- 300 ,(2001) , 10.1016/S0003-2670(00)01283-6
Chifuyu Toriumi, Kazuhiro Imai, Determination of insulin in a single islet of Langerhans by high-performance liquid chromatography with fluorescence detection. Analytical Chemistry. ,vol. 74, pp. 2321- 2327 ,(2002) , 10.1021/AC020043T
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