A fluorimetric, column‐switching HPLC and its application to an elimination study of LLU‐α enantiomers in rat plasma

摘要: A method for the enantiomeric determination of 2,7,8-trimethyl-2-(β-carboxyethyl)-6-hydroxy chroman (LLU-α, γ-CEHC) in rat plasma was developed using high-performance liquid chromatography (HPLC) with a fluorimetric derivatization 4-N, N-dimethylaminosulfonyl-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) followed by O-acetylation acetyl chloride. The proposed HPLC system used two non-chiral columns (phenyl and octadecylsilica) chiral column (a modified cellulose type), which were connected via column-switching valves. derivatized sample prepared from first separated on phenyl column, fraction including LLU-α derivative introduced to octadecylsilica quantify concentration mixture S- R-LLU-α. Finally, directly injected into obtain ratio enantiomers. applied after intravenous administration racemic LLU-α. S-LLU-α eliminated faster than R-LLU-α, its decreased one-third at 2 min dosing. Copyright © 2001 John Wiley & Sons, Ltd. Abbreviations used: DBD-PZ 4-N,N-dimethylaminosulfonyl-7-piperazino-2,1,3-benzoxadiazole DPDS 2,2′-dipyridinyl disulfide LLU-α, γ-CEHC 2,7,8-trimethyl-2-(β-carboxyethyl)-6-hydroxy chroman TFA trifluoroacetic acid TPP triphenylphosphine