A method to evaluate the renin-angiotensin system in rat renal cortex using a microdialysis technique combined with HPLC-fluorescence detection.
作者： Toshi Kajiro , Yuki Nakajima , Takeshi Fukushima , Kazuhiro Imai
DOI: 10.1021/AC020059J
关键词: Detection limit 、 Biochemistry 、 Dialysis tubing 、 Renal cortex 、 Renin–angiotensin system 、 High-performance liquid chromatography 、 Microdialysis 、 Quantitative analysis (chemistry) 、 Chromatography 、 In vivo 、 Chemistry
摘要: A microdialysis (MD) technique, combined with HPLC-fluorescence (FL) detection, was developed for the evaluation of tissue-specific renin−angiotensin system (RAS) in rat renal cortex. An MD probe constructed a hydrophilic hollow fiber dialysis tubing, AN69, showed high recovery (more than 50%) vitro all four angiotensins: angiotensin I (Ang I), Ang II, III, and (1−7). Angiotensins, successfully derivatized m-BS-ABD-F, water-soluble fluorogenic reagent that has 2,1,3-benzoxadiazole (benzofurazan) structure, could be simultaneously determined by coupled-column HPLC. The detection limit I, (1−7) were 94, 44, 47, 83 fmol, respectively. All these peptides good linearity (0.0125−3.1 μM, equivalent to 0.25−62 pmol, correlation coefficient >0.99) precision (recovery >91%). In studies, generation II observed when perfused, major biologically active...
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