Identification of rat urinary kinin as bradykinin.

作者: Yukari Hagiwara , Michiyo Kojima , Tetsuo Kuraishi , Izumi Hayashi , Toshiyuki Miyata

DOI: 10.1016/0024-3205(95)02035-H

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摘要: Bradykinin (BK)-like activity, which was detected by BK-enzyme-immunoassay, purified from 80 ml of ureter urine Sprague-Dawley rats Sephadex G 25 chromatography, FPLC, and reversed phase HPLC. The kinin fraction showed the same retention time as authentic BK on HPLC produced contraction isolated rat uterus, being suppressed a B2-antagonist Hoe140. There no other at corresponding to kallidin, arginyl-BK or T-kinin. peptide an amino acid sequence identical that analysis.

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