Expression of Cdc18/Cdc6 and Cdt1 during G2 phase induces initiation of DNA replication
作者： Stephanie K Yanow , Zoi Lygerou , Paul Nurse
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摘要: Cdc18/Cdc6 and Cdt1 are essential initiation factors for DNA replication. In this paper we show that expression of Cdc18 in fission yeast G2 cells is sufficient to override the controls ensure one S phase per cell cycle. induces synthesis by re-firing replication origins recruiting MCM Cdc21 chromatin presence low levels Cdt1. However, when expressed together with G2, undergo very rapid, uncontrolled synthesis, accumulating contents 64C or more. Our data suggest may potentiate re-replication inducing fire more persistently, possibly stabilizing on chromatin. addition, level a mutant form cannot be phosphorylated cyclin-dependent kinases not induce but does so only co-expressed Thus, regulation both plays crucial role preventing re-initiation until next
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