A cell-based evaluation of a non-essential amino acid formulation as a non-bioactive control for activation and stimulation of muscle protein synthesis using ex vivo human serum

摘要: PurposeThe purpose of this study was to compare the effect treating skeletal muscle cells with media conditioned by postprandial ex vivo human serum fed either isonitrogenous NEAA or a whey protein hydrolysate (WPH) on stimulating MPS in C2C12 cells.nnMethodsBlood taken from six young healthy males following overnight fast (fasted) and 60 min (fed) ingestion WPH (0.33 g.kg-1 Body Mass). myotubes were treated (20%) for 4 h. Activation signalling (phosphorylation mTOR, P70S6K 4E-BP1) determined vitro Western Blot subsequent de novo surface sensing translation technique (SUnSET) techniques, respectively.nnResultsMedia had no compared fasted, whereas significantly increased 4E-BP1 phosphorylation (p<0.01, p<0.05) fasted serum. Furthermore, serum.nnConclusionIn summary, did not result activation MPS. Therefore, these findings suggest use acts as an effective control comparing bioactivity different proteins These also confirm that WPH-fed is primarily due circulating EAA.