Soybean bowman--birk inhibitor conjugates with clinical dextran. synthesis and antiproteolytic activity.

摘要: Conjugates of the classical soybean Bowman–Birk inhibitor (BBI) with clinical dextran were synthesized. Clinical was preliminarily oxidized periodate to dialdehydedextran (DAD). The effect degree oxidation DAD on coupling evaluated. binding protein shown increase increasing (5,10,20%). Total occurred when 20%. BBI–DAD (20%) conjugate contained 13% BBI/DAD molar ratio 1 :1. conjugates retained ability inhibit trypsin (Ki = 0.2-0.3 nM) and α-chymotrypsin 15-30 nM). Thus, BBI polymeric carrier caused practically no decrease in antiproteolytic activity inhibitor.