Pericellular mobilization of the tissue-destructive cysteine proteinases, cathepsins B, L, and S, by human monocyte-derived macrophages.

摘要: Abstract Human macrophages are believed to damage host tissues in chronic inflammatory disease states, but these cells have been reported express only modest degradative activity vitro. However, while examining the ability of human monocytes degrade extracellular matrix component elastin, we identified culture conditions under which matured into a macrophage population that displayed phenotype hundreds times more destructive than previously ascribed any other cell population. The monocyte-derived synthesized elastinolytic metalloproteinases (i.e., gelatinase B and matrilysin) as well cysteine proteinases cathepsins B, L, S), were detected milieu fully processed, mature enzymes by either vital fluorescence or active-site labeling. Consistent with observations, macrophage-mediated was not affected metalloproteinase inhibitors could be almost completely abrogated inhibiting L S. These data demonstrate mobilize arm themselves powerful effector mechanism can participate pathophysiologic remodeling matrix.