Cutting Edge: p21-Activated Kinase (PAK) Is Required for Fas-Induced JNK Activation in Jurkat Cells
作者： Gary M. Bokoch , Thomas Rudel , Tsung-Hsien Chuang , Frank T. Zenke
DOI:
关键词:
摘要: The process of apoptosis is a critical component normal immune system development and homeostasis, in many cells this involves signaling through the c-Jun amino terminal kinase (JNK) pathway. In Jurkat T cells, Fas-induced JNK activity dependent upon activation caspase cascades known to be central components apoptotic program. We show cell lines expressing dominant negative PAK construct that necessary for response Fas receptor cross-linking. Inhibition induced by does not impair death as assessed DNA fragmentation. However, expression catalytically active C terminus PAK2, which generated action during Fas-mediated apoptosis, induces apoptosis. conclude resulting from caspase-mediated cleavage PAK2 can contribute induction death.
参考文章(48)
Arul M. Chinnaiyan, Vishva M. Dixit, Peter H. Krammer, Marcus E. Peter, Michael A. Cahill, Michael A. Cahill, Alfred Nordheim, Frank C. Kischkel, CD95 (APO-1/Fas) induces activation of SAP kinases downstream of ICE-like proteases. Oncogene. ,vol. 13, pp. 2087- 2096 ,(1996)
Shubha Bagrodia, Benoit Dérijard, Roger J. Davis, Richard A. Cerione, Cdc42 and PAK-mediated Signaling Leads to Jun Kinase and p38 Mitogen-activated Protein Kinase Activation Journal of Biological Chemistry. ,vol. 270, pp. 27995- 27998 ,(1995) , 10.1074/JBC.270.47.27995
K Kuida, J. Lippke, G Ku, M. Harding, D. Livingston, M. Su, R. Flavell, Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme Science. ,vol. 267, pp. 2000- 2003 ,(1995) , 10.1126/SCIENCE.7535475
Jonathan Ham, Carol Babij, Jonathan Whitfield, Curt M Pfarr, Dominique Lallemand, Moshe Yaniv, Lee L Rubin, A c-jun dominant negative mutant protects sympathetic neurons against programmed cell death Neuron. ,vol. 14, pp. 927- 939 ,(1995) , 10.1016/0896-6273(95)90331-3
Marcel Verheij, Ron Bose, Xin Hua Lin, Bei Yao, W. David Jarvis, Steven Grant, Michael J. Birrer, Eva Szabo, Leonard I. Zon, John M. Kyriakis, Adriana Haimovitz-Friedman, Zvi Fuks, Richard N. Kolesnick, Requirement for ceramide-initiated SAPK/JNK signalling in stress-induced apoptosis. Nature. ,vol. 380, pp. 75- 79 ,(1996) , 10.1038/380075A0
Shubha Bagrodia, Stephen J. Taylor, Caretha L. Creasy, Jonathan Chernoff, Richard A. Cerione, Identification of a Mouse p21Cdc42/Rac Activated Kinase Journal of Biological Chemistry. ,vol. 270, pp. 22731- 22737 ,(1995) , 10.1074/JBC.270.39.22731
Michael H Cardone, Guy S Salvesen, Christian Widmann, Gary Johnson, Steven M Frisch, The Regulation of Anoikis: MEKK-1 Activation Requires Cleavage by Caspases Cell. ,vol. 90, pp. 315- 323 ,(1997) , 10.1016/S0092-8674(00)80339-6
Z. Xia, M. Dickens, J. l Raingeaud, R. J. Davis, M. E. Greenberg, Opposing Effects of ERK and JNK-p38 MAP Kinases on Apoptosis Science. ,vol. 270, pp. 1326- 1331 ,(1995) , 10.1126/SCIENCE.270.5240.1326
T. S. Griffith, T. Brunner, S. M. Fletcher, D. R. Green, T. A. Ferguson, Fas Ligand-Induced Apoptosis as a Mechanism of Immune Privilege Science. ,vol. 270, pp. 1189- 1192 ,(1995) , 10.1126/SCIENCE.270.5239.1189
I. Nicoletti, G. Migliorati, M.C. Pagliacci, F. Grignani, C. Riccardi, A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry Journal of Immunological Methods. ,vol. 139, pp. 271- 279 ,(1991) , 10.1016/0022-1759(91)90198-O