Bisabololoxide A, one of the main constituents in German chamomile extract, induces apoptosis in rat thymocytes.

摘要: German chamomile (Matricaria recutita L.), one of the popular ingredients in herbal teas, has been traditionally used for medicinal purposes. Bisabololoxide A (BSBO) is main constituents this herb. BSBO supposed to be principle some bioactivities such as anti-inflammatory, gastrointestinal, and antipruritic actions. Although use spread, information related toxicity very limited. In present study, cytotoxic effect micromolar was cytometrically examined on rat thymocytes by using appropriate fluorescent dyes. When cells were incubated with 24 h, at concentrations 30 μM or more significantly increased populations dead cells, shrunken phosphatidylserine exposed membrane surface. Both cell shrinkage externalization are general features an early stage apoptosis. addition, population containing hypodiploid DNA, increase completely attenuated Z-VAD-FMK, a pan-inhibitor caspases, indicating involvement caspase activation. Thus, it likely that type death induced The significant changes cellular parameters not observed when concentration 10 less. Furthermore, short incubation (3 h) even 30–100 did affect cells. Therefore, may suggested practically safe conventionally used.