Synthesis of uridine diphosphate [1-3H]glucose and uridine diphosphate [6-3H]glucose
作者： E. V. Snetkova , G. P. Akulov , L. S. Gordeeva , Yu. L. Kaminskii
DOI: 10.1007/BF00602470
关键词:
摘要: Information is given on the synthesis of tritium-labeled UDPG by successive transformation D-[1-3H]glucose or D-[6-3H]glucose action enzymes hexokinase (EC 2.7.1.1), phosphoglucomutase 2.7.5.1), and UDPG-pyrophosphorylase 2.7.7.9) under conditions ensuring retention tritium label. Methods obtaining tritium-labelled substrates intermediate products their enzymatic transformations in [3H]UDPG are discussed.
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