Macromolecular synthesis, differentiation and cell division in Tetrahymena pyriformis mating type I variety 1

摘要: In Tetrahymena pyriformis, mating type I, variety 1, cycloheximide rapidly and completely inhibited incorporation of 14C-L-leucine into protein. Actinomycin D (25 μg per ml) 14C-uracil cold-TCA-insoluble material, after a 5–10 minute lag. Frequently subsequent decline in the amount radioactivity was observed. Protein synthesis continued actinomycintreated cultures for variable time cessation RNA synthesis. Oral development affected by virtually immediately, actinomycin 10–15 Cells either drug before onset oral membranelle formation were permanently arrested stomatogenic field phase. early middle stages completed membranelles, but did not invariably complete cell division. Cycloheximide, when added at beginning formation, brought about arrest or resorption membranelles they completed. elicit resorption, sometimes blockage during fully formed (and division just beginning) development, nuclear divisions, These results suggest that concurrent protein are essential initiation completion differentiation. The also specific messenger RNA(s) with very short half-life is required proteins involved