Anti-Cancer Effects of Glaucarubinone in the Hepatocellular Carcinoma Cell Line Huh7 via Regulation of the Epithelial-To-Mesenchymal Transition-Associated Transcription Factor Twist1

摘要: Hepatocellular carcinoma (HCC), the most common type of liver cancer, is a leading cause cancer-related deaths. As HCC has high mortality rate and its incidence increasing worldwide, understanding treating are crucial for resolving major public health concerns. In present study, wound healing screening assays were performed using natural product libraries to identify chemicals that can inhibit cancer cell migration. Glaucarubinone (GCB) showed potential inhibiting The anti-cancer effects GCB evaluated line, Huh7. effects, as verified by healing, migration, invasion, colony formation, three-dimensional spheroid invasion assays. addition, cells treated with suppressed matrix metalloproteinase activities. Immunoblotting analyses intracellular signaling pathways revealed regulated levels Twist1, transcription factor associated epithelial-to-mesenchymal transition, mitogen-activated protein kinase. invasive ability was found be decreased regulation Twist1 levels. Furthermore, downregulated phosphorylation extracellular signal-regulated These results indicate exhibits anti-metastatic properties in Huh7 cells, suggesting it could used treat HCC.