Anandamide induces cell death independently of cannabinoid receptors or vanilloid receptor 1: possible involvement of lipid rafts.
作者： K. P. Sarker , I. Maruyama
DOI: 10.1007/S00018-003-3055-2
关键词:
摘要: Anandamide triggers various cellular activities by binding to cannabinboid (CB1/CB2) receptors or vanilloid receptor 1 (VR1). However, the role of these in anandamide-induced apoptosis remains largely unknown. Here, we show that SR141716A, a specific inhibitor cannabinoid (CB1-R), did not block cell death endogenously CB1-R expressing cells. In addition, CB1-R-lacking Chinese hamster ovary (CHO) cells underwent after anandamide treatment. SR144528, CB2-R also failed HL-60 Capsazepine, antagonist VR1 could prevent constitutively and PC12 Moreover, noticeably triggered VR1-lacking human embryonic kidney (HEK) contrast, methyl-β cyclodextrin (MCD), membrane cholesterol depletor, completely blocked variety cells, including PC12, C6, Neuro-2a, CHO, HEK, SMC, Jurkat MCD superoxide generation, phosphatidyl serine exposure p38 MAPK/JNK activation. Thus, our data imply novel for lipid rafts death.
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