High-performance liquid chromatography of carboxylic acids using 4-bromomethyl-7-acetoxycoumarin as fluorescence reagent
作者： Hironori Tsuchiya , Tokishi Hayashi , Hiroshi Naruse , Nobuhiko Takagi
DOI: 10.1016/S0021-9673(00)81786-0
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摘要: Abstract A sytem for the high-performance liquid chromatography of carboxylic acids using 4-bromomethyl-7-acetoxycoumarin (Br-Mac) as fluorescence reagents is described. Br-Mac reacts with to give ester derivatives, which are separated reversed-phase chromatography. The eluate from column mixed an alkaline solution. labelled hydrolysed fluorescent coumarin introduced into a flow-through fluorimeter. In this system, hydrolysate, equimolar acid, detected and fluorophor common every acid. Only slight variation found in peak areas various acids. gradient elution technique effectively used system because quantum yield hydrolysate not affected by constitution mobile phase. Low femtomole levels can be detected.
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