Effect of untranslated leader sequence of AMV RNA 4 and signal peptide of pathogenesis-related protein 1b on attacin gene expression, and resistance to fire blight in transgenic apple

摘要: A cDNA clone of the gene encoding attacin was used to construct three plasmid binary vectors in which attE controlled by cauliflower mosaic virus 35S promoter with duplicated upstream B domain (35S) (p35SAtt), untranslated leader sequence alfalfa RNA 4 (AMV) (p35SAMVAtt), and AMV signal peptide pathogenesis-related protein 1b from tobacco (SP) (p35SAMVSPAtt), respectively. These plasmids pLDB15 containing under control potato proteinase inhibitor II (Pin2) were Agrobacterium-mediated transformation apple scion cultivar `Galaxy' rootstock M.26 enhance resistance Erwinia amylovora, bacterium that causes fire blight. The mean content transgenic lines times higher than without AMV. Northern blots suggested functioned as it does other plant species enhancing translation mRNA. Transgenic fused SP had lower SP. In vitro assays indicated partially degraded intercellular fluid leaves. However, transformed significantly less disease those suggesting intercellularly secreted is more effective reducing E. amylovora infection intracellularly localized attacin. negative correlation observed between Pin2Att following inoculation enhances