Function and glycosylation of plant-derived antiviral monoclonal antibody
作者： K. Ko , Y. Tekoah , P. M. Rudd , D. J. Harvey , R. A. Dwek
关键词: Antibody 、 Alfalfa mosaic virus 、 Rabies virus 、 Biology 、 Molecular biology 、 Glycosylation 、 Immunoglobulin light chain 、 Virology 、 KDEL 、 Monoclonal antibody 、 Antigen
摘要: Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative current methods antibody in animal systems. In this study, heavy light chains human anti-rabies were expressed assembled planta under control two strong constitutive promoters. An alfalfa mosaic virus untranslated leader sequence Lys-Asp-Glu-Leu (KDEL) endoplasmic reticulum retention signal linked at N C terminus chain, respectively. mAbP was as effective neutralizing activity rabies mammalian-derived (mAbM) or Ig (HRIG). The contained mainly oligomannose type N-glycans (90%) had no potentially antigenic α(1,3)-linked fucose residues. shorter half-life than mAbM. efficient HRIG for post-exposure prophylaxis against hamsters, indicating that differences N-glycosylation do not affect efficacy model.
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sci-hub.se 参考文章(44)
Thomas Bouquin, Mads Thomsen, Leif Kofoed Nielsen, Trine Hefsgaard Green, John Mundy, Morten Hanefeld Dziegiel, Human anti-rhesus D IgG1 antibody produced in transgenic plants Transgenic Research. ,vol. 11, pp. 115- 122 ,(2002) , 10.1023/A:1015226418688
Kisung Ko, John L. Norelli, Jean-Paul Reynoird, Ewa Boresjza-Wysocka, Susan K. Brown, Herb S. Aldwinckle, Effect of untranslated leader sequence of AMV RNA 4 and signal peptide of pathogenesis-related protein 1b on attacin gene expression, and resistance to fire blight in transgenic apple Biotechnology Letters. ,vol. 22, pp. 373- 381 ,(2000) , 10.1023/A:1005672601625
Sherie L. Morrison, Ann Wright, Effect of C2-Associated Carbohydrate Structure on Ig Effector Function: Studies with Chimeric Mouse-Human IgG1 Antibodies in Glycosylation Mutants of Chinese Hamster Ovary Cells Journal of Immunology. ,vol. 160, pp. 3393- 3402 ,(1998)
Pauline M. Rudd, Geoffrey R. Gulle, Bernhard Küster, David J. Harvey, Ghislain Opdenakker, Raymond A. Dwek, Oligosaccharide sequencing technology Nature. ,vol. 388, pp. 205- 207 ,(1997) , 10.1038/40677
Larry Zeitlin, Stuart S. Olmsted, Thomas R. Moench, Man Sung Co, Brian J. Martinell, Vikram M. Paradkar, David R. Russell, Cary Queen, Richard A. Cone, Kevin J. Whaley, A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes Nature Biotechnology. ,vol. 16, pp. 1361- 1364 ,(1998) , 10.1038/4344
Udo Conrad, Ulrike Fiedler, Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity. Plant Molecular Biology. ,vol. 38, pp. 101- 109 ,(1998) , 10.1023/A:1006029617949
Jack Black, Hilary Koprowski, Studies on Chick-Embryo-Adapted Rabies Virus. IV. Immunization of Guinea-Pigs and Description of a Potency Control Test. Journal of Immunology. ,vol. 72, pp. 79- 84 ,(1954)
B Dietzschold, M Gore, P Casali, Y Ueki, C E Rupprecht, A L Notkins, H Koprowski, Biological characterization of human monoclonal antibodies to rabies virus. Journal of Virology. ,vol. 64, pp. 3087- 3090 ,(1990) , 10.1128/JVI.64.6.3087-3090.1990
Henry Daniell, Stephen J Streatfield, Keith Wycoff, Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants Trends in Plant Science. ,vol. 6, pp. 219- 226 ,(2001) , 10.1016/S1360-1385(01)01922-7
Janet M. Sharp, Pauline M. Doran, Characterization of monoclonal antibody fragments produced by plant cells. Biotechnology and Bioengineering. ,vol. 73, pp. 338- 346 ,(2001) , 10.1002/BIT.1067