Changes in Glycogen, Pyruvate and Lactate in Rainbow Trout with Bacterial Gill Disease

作者: Hisatsugu WAKABAYASHI , Toshiyuki IWADO

DOI: 10.3147/JSFP.20.161

关键词:

摘要: To test the hypothesis that mortality of bacterial gill disease (BGD) is related to tissue hypoxia, changes in glycogen, pyruvate and lactate concentrations muscle juvenile rainbow trout with BGD were determined. The levels glycogen fish infected experimentally Flavobacterium sp. 7721 (ATCC 35035) showed a progressive decline following infection. This was interpreted as reflection fact listless did not take food. Muscle concentration reached lowest values, 78.6±27.0 mg%, when experimental began turn sideways, then rised 133.0±57.7mg%until they died. increase lactate/pyruvate ratio (L/P ratio) at time death more significant than lactate. appeared reflect accumulation “excess lactate” muscle.We concluded breakdown gas exchange gills caused failure circulation provide oxygen enough remove excess from muscle, even though level so high healthy fish.

参考文章(10)
Theodore E. Friedemann, Gladys E. Haugen, PYRUVIC ACID: II. THE DETERMINATION OF KETO ACIDS IN BLOOD AND URINE Journal of Biological Chemistry. ,vol. 147, pp. 415- 442 ,(1943) , 10.1016/S0021-9258(18)72397-1
S.B. Barker, William H. Summerson, The colorimetric determination of lactic acid in biological material. Journal of Biological Chemistry. ,vol. 138, pp. 535- 554 ,(1941) , 10.1016/S0021-9258(18)51379-X
Edgar C. Black, Anne Robertson Connor, Kwok-Cheung Lam, Wing-Gay Chiu, Changes in Glycogen, Pyruvate and Lactate in Rainbow Trout Salmo gairdneri) During and Following Muscular Activity Wsq: Women's Studies Quarterly. ,vol. 19, pp. 409- 436 ,(1962) , 10.1139/F62-024
William E. Huckabee, RELATIONSHIPS OF PYRUVATE AND LACTATE DURING ANAEROBIC METABOLISM. II. EXERCISE AND FORMATION OF O2-DEBT1 Journal of Clinical Investigation. ,vol. 37, pp. 255- 263 ,(1958) , 10.1172/JCI103604
H. Wakabayashi, S. Egusa, J. L. Fryer, Characteristics of Filamentous Bacteria Isolated from a Gill Disease of Salmonids Canadian Journal of Fisheries and Aquatic Sciences. ,vol. 37, pp. 1499- 1504 ,(1980) , 10.1139/F80-194
William E. Huckabee, RELATIONSHIPS OF PYRUVATE AND LACTATE DURING ANAEROBIC METABOLISM. III. EFFECT OF BREATHING LOW-OXYGEN GASES1 Journal of Clinical Investigation. ,vol. 37, pp. 264- 271 ,(1958) , 10.1172/JCI103605
Rex Montgomery, Determination of glycogen Archives of Biochemistry and Biophysics. ,vol. 67, pp. 378- 386 ,(1957) , 10.1016/0003-9861(57)90292-8
Norihiko KIMURA, Hisatsugu WAKABAYASHI, Shigeharu KUDO, Studies on Bacterial Gill Disease in Salmonids—I Fish Pathology. ,vol. 12, pp. 233- 242 ,(1978) , 10.3147/JSFP.12.233
Jacob Sacks, Richard V. Ganslen, Joseph T. Diffee, Lactic and Pyruvic Acid Relations in Frog Muscle American Journal of Physiology-Legacy Content. ,vol. 177, pp. 113- 114 ,(1954) , 10.1152/AJPLEGACY.1954.177.1.113