Chapter 2 Multiphase chemistry of cell injury

摘要: Summary Living cells are in an unstable state, responding often rapidly to gentle physiological stimuli. To study them either after the slow fixation and dehydration required for electron microscopy or by homogenizing ensures that resulting their components virtually unable demonstrate effects of normal trauma low concentrations toxic substances hormones. Multiphase chemistry has been designed allow investigation cellular biochemistry biophysics without such disturbances. This development a special form quantitative biochemistry. Where it possible compare results with conventional methods, there good agreement. But whereas biochemical procedures require 10 6 each sample, multiphase selects 20 even complex tissue. The advantages this shown number studies; on problems arose venous transplants; cardiac surgery; very sensitive micro-bioassays (10 −12 g/ml) polypeptide