Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma
作者： CJ de Boer , E Schuuring , E Dreef , G Peters , J Bartek
DOI: 10.1182/BLOOD.V86.7.2715.2715
关键词:
摘要: Mantle cell lymphoma (MCL) is a clinicopathologic entity that difficult to diagnose on histopathologic criteria. Approximately 50% 70% of MCL contain t(11;14)(q13;q32) translocation involving the cyclin D1 gene. Irrespective this rearrangement, almost all show overexpression gene at mRNA level. Other B-cell non-Hodgkin's lymphomas (NHL) do not rearrangement or D1. We developed an immunohistochemical assay detect protein conventional formalin-fixed, paraffin-embedded biopsies using well-defined monoclonal antibody DCS-6. Expression in tumor cells was compared with expression endothelial and fibroblasts. An exclusively nuclear staining pattern observed. Moreover, directly observed by immunoblot analysis same antibody, as well occurrence genomic rearrangements within BCL-1 locus. Of 13 were analyzed immunohistochemistry immunoblot, 12 showed both techniques, whereas no 39 other NHL. additional studied either 11 also overexpression. Two morphologically indistinguishable from but aberrant immunophenotype (CD5 negative, CD10 positive) lacked These results underscore significance specific marker for MCL. Detection tissues DCS-6 can be applied routine diagnostic purposes.
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ashpublications.org参考文章(42)
T Motokura, W I Yang, N L Harris, L R Zukerberg, A Arnold, Cyclin D1 (Bcl-1, PRAD1) protein expression in low-grade B-cell lymphomas and reactive hyperplasia. American Journal of Pathology. ,vol. 145, pp. 86- 96 ,(1994)
Parry D, Dickson C, Peters G, Holder A, Bates S, MacAllan D, Bonetta L, CDK6 (PLSTIRE) and CDK4 (PSK-J3) are a distinct subset of the cyclin-dependent kinases that associate with cyclin D1. Oncogene. ,vol. 9, pp. 71- 79 ,(1994)
F Berger, P Felman, A Sonet, G Salles, Y Bastion, PA Bryon, B Coiffier, Nonfollicular small B-cell lymphomas: a heterogeneous group of patients with distinct clinical features and outcome Blood. ,vol. 83, pp. 2829- 2835 ,(1994) , 10.1182/BLOOD.V83.10.2829.2829
R. M. Santella, Yu-Jing Zhang, S. Doi, A. S. Infante, S. M. Kahn, I. B. Weinstein, A. M. Cacace, Wei Jiang, Ping Zhou, Overexpression of cyclin D1 in rat fibroblasts causes abnormalities in growth control, cell cycle progression and gene expression. Oncogene. ,vol. 8, pp. 3447- 3457 ,(1993)
D Jadayel, E Matutes, MJ Dyer, V Brito-Babapulle, MT Khohkar, D Oscier, D Catovsky, Splenic lymphoma with villous lymphocytes: analysis of BCL-1 rearrangements and expression of the cyclin D1 gene. Blood. ,vol. 83, pp. 3664- 3671 ,(1994) , 10.1182/BLOOD.V83.12.3664.3664
Palmero I, Sinclair Aj, Peters G, Holder A, Dickson C, Cyclins D1 and D2 are differentially expressed in human B-lymphoid cell lines. Oncogene. ,vol. 8, pp. 1049- 1054 ,(1993)
Michele Pagano, Jiri Lukas, Jiri Bartek, Giulio Draetta, Zdenka Staskova, Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines. Oncogene. ,vol. 9, pp. 707- 718 ,(1994)
Michele Pagano, Anne M. Theodoras, Jerry W. Shay, Sun W. Tam, Giulio F. Draetta, Differential expression and regulation of Cyclin D1 protein in normal and tumor human cells: association with Cdk4 is required for Cyclin D1 function in G1 progression. Oncogene. ,vol. 9, pp. 2663- 2674 ,(1994)
M Hummel, J Tamaru, B Kalvelage, H Stein, Mantle cell (previously centrocytic) lymphomas express VH genes with no or very little somatic mutations like the physiologic cells of the follicle mantle. Blood. ,vol. 84, pp. 403- 407 ,(1994) , 10.1182/BLOOD.V84.2.403.403
J. Bartkova, E. Nacheva, J. Lukas, M.J.S. Dyer, J. Bartek, M. Strauss, D. Jadayel, BCL-1/cyclin D1 oncoprotein oscillates and subverts the G1 phase control in B-cell neoplasms carrying the t(11;14) translocation. Oncogene. ,vol. 9, pp. 2159- 2167 ,(1994)