Infection mechanisms of Botrytis species: pre-penetration and pre-infection processes of dry and wet conidia

摘要: Light and electron microscope studies of the infection mechanisms two species Botrytis, B. cinerea fabae, axe described. Conidia were inoculated dry or in presence aqueous glucose onto glass Vicia faba leaves incubated a moist chamber. In all cases, dry-inoculated conidia germinated rapidly to produce short germ-tubes only. contrast, glucose, long which extended across substratum. Low temperature SEM confirmed these results showed further that enclosed by an extensive sheath fibrillar-like material. No such matrix material was found be associated with conidia. Immunofluorescence microscopy immunogold labelling ultrathin sections fabae-infected bean carried out using Botrytis-specific monoclonal antibody, BC-KH4. every case antibody-binding observed at surface both wet Furthermore, when also located throughout germ-tubes. At TEM level, traces amorphous site penetration leaf surface. This immunolabelled Low-temperature macroscopic lesions formed on Botrytis revealed corresponded clearly defined areas collapsed epidermal cells.