Development and evaluation of a rapid neutralizing antibody test for rabies.

作者: Seiji Shiota , Kazuaki Mannen , Takashi Matsumoto , Kentaro Yamada , Takehito Yasui

DOI: 10.1016/J.JVIROMET.2009.05.018

关键词:

摘要: The level of virus-neutralizing antibody, which plays a crucial role in the prevention rabies, is determined by rabies virus (RABV) neutralizing test, are time- and cost-consuming. In order to determine antibody vaccinees, an easy reliable method needed. Based on principle immunochromatography, we developed RAPINA (RAPId Neutralizing Antibody) test presence serum. if equivalent 0.5 IU/ml serum sample mixed with optimal amount inactivated RABV (iRABV) completely absorbed virus, none iRABV can bind monoclonal that recognizes glycoprotein (G) strip. A total 115 human sera samples were tested. sensitivity, specificity accuracy compared rapid fluorescent focus inhibition (RFFIT) as standard 88.7, 91.9 90.4%, respectively. simple, safe method, be substitute for tests use live viruses, cultured cells fluorescence microscopy. This might useful screening large number sera.

参考文章(15)
Pamela A. Yager, George M. Baer, Jean S. Smith, A rapid reproducible test for determining rabies neutralizing antibody Bulletin of The World Health Organization. ,vol. 48, pp. 535- 541 ,(1973)
J H Cox, B Dietzschold, L G Schneider, Rabies virus glycoprotein. II. Biological and serological characterization. Infection and Immunity. ,vol. 16, pp. 754- 759 ,(1977) , 10.1128/IAI.16.3.754-759.1977
Akira Nishizono, Pakamatz Khawplod, Kamruddin Ahmed, Kazuyo Goto, Seiji Shiota, Kumato Mifune, Takehito Yasui, Katsuyoshi Takayama, Yukuharu Kobayashi, Kazuaki Mannen, Veera Tepsumethanon, Chanarong Mitmoonpitak, Satoshi Inoue, Kinjiro Morimoto, A simple and rapid immunochromatographic test kit for rabies diagnosis Microbiology and Immunology. ,vol. 52, pp. 243- 249 ,(2008) , 10.1111/J.1348-0421.2008.00031.X
Seiji Shiota, Pakamatz Khawplod, Kamruddin Ahmed, Kumato Mifune, Akira Nishizono, A pilot study on intradermal vaccination of Japanese rabies vaccine for pre-exposure immunization Vaccine. ,vol. 26, pp. 6441- 6444 ,(2008) , 10.1016/J.VACCINE.2008.08.068
T. Ogawa, K. Gamoh, H. Aoki, R. Kobayashi, M. Etoh, M. Senda, N. Hirayama, M. Nishimura, R. Shiraishi, A. Servat, F. Cliquet, Validation and Standardization of Virus Neutralizing Test Using Indirect Immunoperoxidase Technique for the Quantification of Antibodies to Rabies Virus Zoonoses and Public Health. ,vol. 55, pp. 323- 327 ,(2008) , 10.1111/J.1863-2378.2008.01128.X
Yohko T. Arai, Mikio Kimura, Yoshihiro Sakaue, Atsuo Hamada, Ken-Ichiro Yamada, Mikio Nakayama, Tomohiko Takasaki, Ichiro Kurane, Antibody responses induced by immunization with a Japanese rabies vaccine determined by neutralization test and enzyme-linked immunosorbent assay. Vaccine. ,vol. 20, pp. 2448- 2453 ,(2002) , 10.1016/S0264-410X(02)00188-3