作者: Hoai-Nam Nguyen , Charles Kervrann , Cyril Cauchois , Vincent Paveau
DOI: 10.1109/ISBI.2015.7164147
关键词:
摘要: Tissue core de-arraying is one of the most important steps in tissue microarray (TMA) image analysis. However, few solutions and mathematical frameworks are available. This paper presents a robust TMA method adapted for digital images from classical optical new fluorescent devices. The proposed algorithm composed three modules: (a) detection, (b) segmentation, (c) array indexing. detection cores performed by local adaptive thresholding isotropic wavelet transform coefficients. segmentation component uses parametric ellipse to delineate boundaries potential cores. Array indices each computed using thin-plate splines estimate deformation deposited grid. Our appropriate non-linear able quantify grids when compared existing algorithms.