Formulation of novel polymer coated iron oxide nanoparticles

摘要: The aim of this study was to investigate how produce iron oxide nanoparticles, with the potential for long circulation times or ability preferentially reach particular tissues. The preparation nanoparticles achieved using inorganic solution methods prepare particles small size a narrow distribution. were coated dextran and carboxymethyl as reference materials same method in nanoparticles. This project investigated use biodegradable polymer poly(glycerol adipate) (PGA) coating nanoparticles. PGA is already used drug delivery systems showed an control rate release drug. can be readily modified pendant functional groups leading modifications physicochemical properties polymer. It also form copolymers hydrophilic poly(ethylene glycol) (PEG). 40% acylated stearic acid (PGA 40%C18) PEGylated copolymer PEG–PGA 40%C18 synthesised work. The formulation polymers. process optimised producing measured by TEM best sizes are (16 ± 5 nm while with, 23 ±7 16 4 diameter). PGA–IONPs over-coated incubation albumin Tween. characterised DLS, zeta potential, transmission electron microscopy. colloidal stability various particle formulations increasing salt concentrations. These demonstrated that PGA–coated more stable than existing formulations, increased obtained overcoating A further increase seen PEG-PGA nanoparticles. The cellular uptake RBITC labelled nanoparticle studied on C6 medulloblastoma cell line monolayer 3-D aggregate cultures fluorescence microscopy, confocal microscopy (TEM) flow cytometry. results indicated these internalised cells, but unusual subcellular Uptake dependent both concentration time. cells metabolised lost from over period 4–12 hours. studies that, after 1 hour, found all compartments, route entry into could not determined. Experiments taken up aggregates, penetrating deep aggregates. Overall, novel well-defined layers, which against aggregation under physiological conditions. show promise variety medical applications.