Comparison of in-house and commercial real time-PCR based carbapenemase gene detection methods in Enterobacteriaceae and non-fermenting gram-negative bacterial isolates.

摘要: Carbapenemase-producing gram-negative bacteria are increasing globally and have been associated with outbreaks in hospital settings. Thus, the accurate detection of these infections is mandatory for administering adequate therapy infection control measures. This study aimed to establish evaluate a multiplex real-time PCR assay simultaneous carbapenemase gene variants rods compare performance commercial RT-PCR (Check-Direct CPE). 116 carbapenem-resistant Enterobacteriaceae, Pseudomonas aeruginosa Acinetobacter baumannii isolates were genotyped genes by sequencing. The defined used validation in-house use designed primer pairs probes. Among bla KPC, VIM, NDM or OXA detected. Both assays detected all VIM isolates. 53 67 (79.0%) whereas only 29 (43.3%) genes. sufficiently distinguished most prevalent types (23-like 48-like) melting curve analysis direct from positive blood culture vials. Check-Direct CPE carbapenem resistance solid Moreover, enabled identification directly blood-culture However, we observed insufficient various both assays. Nevertheless, majority type Enterobacteriaceae A. baumannii.