The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury

摘要: Chronic liver diseases, such as viral hepatitis, alcoholic disease, or non-alcoholic fatty are characterized by continual inflammation, progressive destruction and regeneration of the hepatic parenchyma, progenitor cell proliferation, fibrosis. The end-stage every chronic disease is cirrhosis, a major risk factor for development hepatocellular carcinoma. To study processes regulating initiation, establishment, progression, several animal models used in laboratories. Here we describe six-week time course choline-deficient ethionine-supplemented (CDE) mouse model, which involves feeding old male C57BL/6J mice with chow 0.15% DL-ethionine-supplemented drinking water. Monitoring health typical body weight loss curve explained. protocol demonstrates gross examination CDE-treated blood collection cardiac puncture subsequent serum analyses. Next, perfusion technique different lobes standard evaluations shown, including histology assessments hematoxylin eosin Sirius Red stainings, immunofluorescent detection populations well transcriptome profiling microenvironment. This model suitable studying inflammatory, fibrogenic, dynamics induced through can be to test potential therapeutic agents that may modulate these processes.