Bovine serum albumin: survival and osmolarity effect in bovine spermatozoa stored above freezing point.
作者： C. F. Nang , K. Osman , S. B. Budin , M. I. Ismail , F. H. F. Jaffar
DOI: 10.1111/J.1439-0272.2011.01203.X
关键词:
摘要: Liquid nitrogen preservation in remote farms is a limitation. The goal of this study was to determine optimum temperature above freezing point for bovine spermatozoa using serum albumin (BSA) as supplementation. Pooled semen sample from three ejaculates subjected various BSA concentration (1, 4, 8 and 12 mg ml(-1)), before incubation different temperatures (4, 25 37 °C). Viability assessment carried out against time day 0 (fresh sample) until all become nonviable. Optimal condition storage at 4 °C with 1 ml(-1) almost 7 days. improved viability declining rate 44.28% 57.59% compared control, 80.54% 98.57% respectively. Increase did not improve sperm viability. Our results also confirmed that there strong negative correlation between media osmolarity survival r = 0.885, P < 0.0001. Bovine helps stored point.
sci-hub.se 参考文章(21)
Bailey Jl, Lessard C, Sullivan R, Parent S, Leclerc P, Cryopreservation alters the levels of the bull sperm surface protein P25b. Journal of Andrology. ,vol. 21, pp. 700- 707 ,(2000) , 10.1002/J.1939-4640.2000.TB02138.X
Mariola Słowińska, Halina Karol, Andrzej Ciereszko, Comet assay of fresh and cryopreserved bull spermatozoa. Cryobiology. ,vol. 56, pp. 100- 102 ,(2008) , 10.1016/J.CRYOBIOL.2007.10.176
B Ozmen, GS Caglar, F Koster, B Schopper, K Diedrich, S Al-Hasani, Relationship between sperm DNA damage, induced acrosome reaction and viability in ICSI patients. Reproductive Biomedicine Online. ,vol. 15, pp. 208- 214 ,(2007) , 10.1016/S1472-6483(10)60710-9
P. Shannon, R. Vishwanath, The effect of optimal and suboptimal concentrations of sperm on the fertility of fresh and frozen bovine semen and a theoretical model to explain the fertility differences Animal Reproduction Science. ,vol. 39, pp. 1- 10 ,(1995) , 10.1016/0378-4320(95)01376-B
WM Maxwell, S Salamon, Liquid storage of ram semen: a review. Reproduction, Fertility and Development. ,vol. 5, pp. 613- 638 ,(1993) , 10.1071/RD9930613
Ranjan Sitaula, Heidi Elmoazzen, Mehmet Toner, Sankha Bhowmick, Desiccation tolerance in bovine sperm: a study of the effect of intracellular sugars and the supplemental roles of an antioxidant and a chelator. Cryobiology. ,vol. 58, pp. 322- 330 ,(2009) , 10.1016/J.CRYOBIOL.2009.03.002
E. Cabrita, V. Robles, S. Cuñado, J.C. Wallace, C. Sarasquete, M.P. Herráez, Evaluation of gilthead sea bream, Sparus aurata, sperm quality after cryopreservation in 5 ml macrotubes☆ Cryobiology. ,vol. 50, pp. 273- 284 ,(2005) , 10.1016/J.CRYOBIOL.2005.02.005
M. Rossato, G. Balercia, G. Lucarelli, C. Foresta, F. Mantero, Role of seminal osmolarity in the regulation of human sperm motility International Journal of Andrology. ,vol. 25, pp. 230- 235 ,(2002) , 10.1046/J.1365-2605.2002.00353.X
Taiki MATSUOKA, Hiroko IMAI, Hirohide KOHNO, Yutaka FUKUI, Effects of bovine serum albumin and trehalose in semen diluents for improvement of frozen-thawed ram spermatozoa. Journal of Reproduction and Development. ,vol. 52, pp. 675- 683 ,(2006) , 10.1262/JRD.18033
Z. Liu, R.H. Foote, Bull Sperm Motility and Membrane Integrity in Media Varying in Osmolality Journal of Dairy Science. ,vol. 81, pp. 1868- 1873 ,(1998) , 10.3168/JDS.S0022-0302(98)75757-1