Fibronectin: purification, immunochemical properties, and biological activities.

摘要: Publisher Summary Fibronectin is a cell-surface and blood glycoprotein that apparently mediates adhesion of cells to the extracellular matrix. Malignant tend lack cell surface fibronectin, this may contribute their capacity for invasive metastatic growth. Early isolation methods plasma fibronectin made use its propensity precipitate with fibrinogen some other proteins when allowed stand in cold. A large fraction cryoprecipitate it can be purified from such by using combination differential precipitation steps ion exchange chromatography. cultured fibroblasts isolated extraction low concentrations urea. Antifibronectin columns also used isolate both cultures. These have now largely been replaced affinity chromatography on gelatin-Sepharose. The immunochemical quantitation include radioimmunoassay, enzyme immunoassay, rocket immunoelectrophoresis. Of these methods, linked immunosorbent assay probably most useful one.