Human Cytomegalovirus-Encoded G Protein-Coupled Receptor UL33 Facilitates Virus Dissemination via the Extracellular and Cell-to-Cell Route.
作者： Jeffrey R. van Senten , Maarten P. Bebelman , Puck van Gasselt , Nick D. Bergkamp , Jelle van den Bor
DOI: 10.3390/V12060594
关键词:
摘要: Human cytomegalovirus (HCMV) encodes four G protein-coupled receptor (GPCR) homologs. Three of these receptors, UL78, US27 and US28, are known for their roles in HCMV dissemination latency. Despite importance its rodent orthologs viral replication pathogenesis, such a function is not reported the HCMV-encoded GPCR UL33. Using clinical strain Merlin, we show that UL33 facilitates both cell-associated cell-free virus transmission. A UL33-deficient derivative revealed retarded spread, formation less smaller plaques, reduced extracellular progeny during multi-cycle growth analysis fibroblast cultures compared to parental virus. The UL33-revertant, US28-deficient, US28-revertant viruses were similar under multistep conditions. UL33- US28-deficient Merlin impaired spread degree. Thus, defect displayed by but reflects UL33’s contribution In conclusion, cultures.
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