Thyroid Antibodies in Spontaneous Autoimmune Thyroiditis in the Buffalo Rat

摘要: Thyroid antibodies in the sera of BUF rats are closely correlated with spontaneous thyroiditis; their detection may facilitate study this animal model organspecific autoimmunity. In a group 115 retired breeders (females older than 1 year), 26% had mononuclear cell infiltration thyroid and high titers detectable by indirect immunofluorescence (IF) chromic chloride passive hemagglutination (CCH). contrast, low-titered were present 9% that normal thyroids. Sequential studies performed on 76 neonatally thymectomized showed at 2 months 24% IF 8% CCH 3 these percentages increased to 27% 25% CCH. When sacrificed 4 months, time when disease is not seen untreated animals, found have thyroids. Approximately 75% thyroiditis been positive for 90% months. At sacrifice all animals high-titered antigens. 36% without thyroiditis. Intravenous injection thymus cells into failed reduce incidence antibodies. 33% both serum antibodies, whereas 19% those thyroids antibodies. Titers circulating initial intermediate stages thyroiditis, i.e., less lowest titers, levels antibody titers. On other hand, degree relatively lower Direct infiltrated revealed presence rat immunoglobulins organs, suggesting possible direct or role autoantibodies pathogenesis disease. We attempted detect delayed hypersensitivity skin testing antigens observing reactions 4, 24, 48 hr. All 123 negative, 20% which No MIF activity was detected The absence experiments provides further support contention be mediated.