Identification and Characterization of a New Enterotoxin Produced by Clostridium perfringens Isolated from Food Poisoning Outbreaks

摘要: There is a strain of Clostridium perfringens, W5052, which does not produce known enterotoxin. We herein report that the W5052 expressed homologue iota-like toxin components sa and sb C. spiroforme, named perfringens enterotoxin, CPILE-a CPILE-b, respectively, based on results genome sequencing analysis systematic protein screening. In nicotinamide glyco-hydrolase (NADase) assay hydrolysis activity was dose-dependently increased by concentration rCPILE-a, as judged mass spectrometry analysis. addition, actin monomer lysates Vero L929 cells were radiolabeled in presence [32P]NAD rCPILE-a. These findings indicated possesses ADP-ribosylation activity. The culture supernatant facilitated rounding killing cells, but rCPILE-a or non-proteolyzed rCPILE-b did not. However, trypsin-treated did. Moreover, mixture enhanced cell activities, compared with induced alone. injection into an ileum loop rabbits evoked swelling accumulation fluid dose-dependently, suggesting CPILE enterotoxic evidence presented this communication will facilitate epidemiological, etiological, toxicological studies food poisoning, also stimulate transfer toxins’ gene(s) among Genus Clostridium.