Effects of tissue fixation conditions and protease pretreatment on immunohistochemical performance of a large series of new anti-keratin monoclonal antibodies: value in oncopathology.

摘要: A comparative study with 21 recently raised monoclonal antibodies (3 of which are reported here for the first time) to human keratin polypeptides was performed on a wide range paraffin-embedded tissues and tumors, aimed at examination effects four different fixatives protease pretreatment immunohistochemical detection keratins. Our data demonstrated that: (a) formaldehyde-based modified by acidification and/or addition methanol gave results superior those achieved routinely used formol saline; (b) relatively rare (4 out 21) could be identified reliable immunostaining patterns even routine formalin-fixed material; (c) proteolytic digestion step preceding beneficial performance majority in our panel. Additional options potentially lead further improvement immunohistochemistry paraffin embedded specimens also suggested. This work provides necessary basis wider application anti-keratin C-series both oncopathology research-oriented retrospective studies.