Expression of foreign genes in sunflower (Helianthus annuus L.) — evaluation of three gene transfer methods

作者: Hélène Laparra , Monique Burrus , Reiner Hunold , Brigitte Damm , Ana-Maria Bravo-Angel

DOI: 10.1007/978-94-011-0357-2_7

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摘要: Suitable sunflower tissues and cells were transformed either by direct gene transfer into protoplasts, particle bombardment, or Agrobacterium co-culture. While all techniques allowed efficient short-term transient expression of the introduced gene(s) in respective tissues, stable transformation was only observed after with Agrobacterium. The latter technique suitable for production transgenic callus from seedling cotyledons occasional shoots chimaeric transgene. Detailed analysis interaction this explant showed that infection efficiency critically dependent on co-culture conditions, preferentially-transformed not ones competent regeneration.

参考文章(32)
S�ren Knudsen, Martin M�ller, Transformation of the developing barley endosperm by particle bombardment. Planta. ,vol. 185, pp. 330- 336 ,(1991) , 10.1007/BF00201052
Nathalie Knittel, V�ronique Gruber, G�nther Hahne, Philippe L�n�e, Transformation of sunflower (Helianthus annuus L.): a reliable protocol. Plant Cell Reports. ,vol. 14, pp. 81- 86 ,(1994) , 10.1007/BF00233766
I. Veidt, S.E. Bouzoubaa, R.-M. Leiser, V.A. Ziegler-Graff, H. Guilley, K. Richards, G. Jonard, Synthesis of full-length transcripts of beet western yellows virus RNA: Messenger properties and biological activity in protoplasts Virology. ,vol. 186, pp. 192- 200 ,(1992) , 10.1016/0042-6822(92)90073-X
G. Vancanneyt, R. Schmidt, A. O'Connor-Sanchez, L. Willmitzer, M. Rocha-Sosa, Construction of an intron-containing marker gene : splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation Molecular Genetics and Genomics. ,vol. 220, pp. 245- 250 ,(1990) , 10.1007/BF00260489
Jean-Luc Jung, Salah Bouzoubaa, David Gilmer, G�nther Hahne, Visualisation of transgene expression at the single protoplast level. Plant Cell Reports. ,vol. 11, pp. 346- 350 ,(1992) , 10.1007/BF00233363
Chris Scelonge, Joanie Martich, Monique Burrus, Lynn Sims, Gary Huffman, Dennis Bidney, Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens. Plant Molecular Biology. ,vol. 18, pp. 301- 313 ,(1992) , 10.1007/BF00034957
K.E. Paterson, N.P. Everett, Regeneration of Helianthus annuus inbred plants from callus Plant Science. ,vol. 42, pp. 125- 132 ,(1985) , 10.1016/0168-9452(85)90152-9
David Twell, Theodore M. Klein, Michael E. Fromm, Sheila McCormick, Transient expression of chimeric genes delivered into pollen by microprojectile bombardment. web science. ,vol. 91, pp. 1270- 1274 ,(1989) , 10.1104/PP.91.4.1270
R. KAY, A. CHAN, M. DALY, J. MCPHERSON, Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes Science. ,vol. 236, pp. 1299- 1302 ,(1987) , 10.1126/SCIENCE.236.4806.1299
R. Hunold, R. Bronner, G. Hahne, Gus Expression in Sunflower Following Microprojectile Bombardment Biotechnology & Biotechnological Equipment. ,vol. 7, pp. 91- 95 ,(1993) , 10.1080/13102818.1993.10818713