作者: Hélène Laparra , Monique Burrus , Reiner Hunold , Brigitte Damm , Ana-Maria Bravo-Angel
DOI: 10.1007/978-94-011-0357-2_7
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摘要: Suitable sunflower tissues and cells were transformed either by direct gene transfer into protoplasts, particle bombardment, or Agrobacterium co-culture. While all techniques allowed efficient short-term transient expression of the introduced gene(s) in respective tissues, stable transformation was only observed after with Agrobacterium. The latter technique suitable for production transgenic callus from seedling cotyledons occasional shoots chimaeric transgene. Detailed analysis interaction this explant showed that infection efficiency critically dependent on co-culture conditions, preferentially-transformed not ones competent regeneration.