Visualisation of transgene expression at the single protoplast level.
作者： Jean-Luc Jung , Salah Bouzoubaa , David Gilmer , G�nther Hahne
DOI: 10.1007/BF00233363
关键词:
摘要: … of the enzymatic activity of the transgenic protein. We describe here a … quinoa protoplasts with viral RNAs, and the expression of the NPT II gene in protoplasts isolated from transgenic …
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sci-hub.se 参考文章(9)
B. Spörlein, A. Mayer, G. Dahlfeld, H.U. Koop, A microassay for quantitative determination of β-glucuronidase reporter gene activities in individually selected single higher plant cells Plant Science. ,vol. 78, pp. 73- 80 ,(1991) , 10.1016/0168-9452(91)90163-3
I. Veidt, S.E. Bouzoubaa, R.-M. Leiser, V.A. Ziegler-Graff, H. Guilley, K. Richards, G. Jonard, Synthesis of full-length transcripts of beet western yellows virus RNA: Messenger properties and biological activity in protoplasts Virology. ,vol. 186, pp. 192- 200 ,(1992) , 10.1016/0042-6822(92)90073-X
L. Ouillet, H. Guilley, G. Jonard, K. Richards, In vitro synthesis of biologically active beet necrotic yellow vein virus RNA. Virology. ,vol. 172, pp. 293- 301 ,(1989) , 10.1016/0042-6822(89)90131-1
Peter J.M. Rottier, Geertje Rezelman, Albert Van Kammen, The inhibition of cowpea mosaic virus replication by actinomycin D. Virology. ,vol. 92, pp. 299- 309 ,(1979) , 10.1016/0042-6822(79)90135-1
Jean Grosset, Yves Meyer, Yvette Chartier, Serge Kauffmann, Michel Legrand, Bernard Fritig, Tobacco Mesophyll Protoplasts Synthesize 1,3-β-Glucanase, Chitinases, and “Osmotins” during in Vitro Culture Plant Physiology. ,vol. 92, pp. 520- 527 ,(1990) , 10.1104/PP.92.2.520
Yoshiaki Otsuki, Itaru Takebe, Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts Virology. ,vol. 38, pp. 497- 499 ,(1969) , 10.1016/0042-6822(69)90167-6
Marc Lepetit, Martine Ehling, Claude Gigot, G�nther Hahne, An internal standard improves the reliability of transient expression studies in plant protoplasts Plant Cell Reports. ,vol. 10, pp. 401- 405 ,(1991) , 10.1007/BF00232611
Yuichiro Watanabe, Tetsuo Meshi, Yoshimi Okada, Infection of tobacco protoplasts with in vitro transcribed tobacco mosaic virus RNA using an improved electroporation method FEBS Letters. ,vol. 219, pp. 65- 69 ,(1987) , 10.1016/0014-5793(87)81191-2
U. K. LAEMMLI, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Nature. ,vol. 227, pp. 680- 685 ,(1970) , 10.1038/227680A0