The Product of the Ricemyb7Unspliced mRNA Dimerizes with the Maize Leucine Zipper Opaque2 and Stimulates Its Activity in a Transient Expression Assay
作者： Franca Locatelli , Marcella Bracale , Flavio Magaraggia , Franco Faoro , Lucia A. Manzocchi
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摘要: myb7 mRNA is present in rice spliced and unspliced forms, splicing being enhanced by anoxia. The protein (Mybleu) encoded the composed of an incomplete Myb domain followed a leucine zipper; however, it lacks canonical sequences for DNA binding, transcriptional activation, nuclear localization. We show here that transiently transformed tobacco protoplasts, Mybleu able to enhance activity maize zipper Opaque2 on its target b32 promoter. transactivation effect strictly dependent presence driven Mybleu-Opaque2 heterodimers. located nucleus, both protoplasts. In rice, expressed regions corresponding undifferentiated cells roots coleoptiles. Therefore, encodes, depending splicing, two transcription factors belonging separate classes. One them, Mybleu, has novel structural characteristics, suggesting existence new mechanisms acting activation transcription.
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