In vitro inactivation of bacterial endotoxin by human lipoproteins and apolipoproteins.

摘要: A chromogenic Limulus amebocyte lysate assay was used to measure the recovery of 1 endotoxin unit per ml. Purified human high-density lipoprotein, low-density and apolipoprotein A1 (apo A1) at a maximum concentration g protein liter reduced less than 40% baseline in both dose- time-dependent manner absence other serum components. Furthermore, lapine fever response dose ml 5-ng/ml kg by greater 0.5 degrees C (P 0.005) when solution preincubated vitro with apo liter. By test, 0.01 B B) (which contained deoxycholate, known endotoxin-disaggregating agent) 0% but not manner. In heat-inactivated (56 C, h) normal serum, lipoprotein cholesterol 0.05) correlated inversely recovery, but, paradoxically, directly 0.05), while showed no significant correlation. INTRALIPID alone had effect on recovery. Addition 10 0.0042 increased from approximately 30 80% 0.001), addition 0.25 decreased 20% 0.001). We conclude that (i) lipoproteins are inactivators; (ii) this ability may be modulated their lipid component (lipid-endotoxin interaction); (iii) is capable inactivating (protein-endotoxin interaction).