Hair follicle development and related gene and protein expression of skins in Rex rabbits during the first 8 weeks of life
作者： Zhenyu Wu , Liangzhan Sun , Gongyan Liu , Hongli Liu , Hanzhong Liu
DOI: 10.5713/AJAS.18.0256
关键词:
摘要: Objective We aimed to observe hair follicle (HF) development in the dorsal skin and elucidate expression patterns of genes proteins related HF Rex rabbits from birth 8 weeks age. Methods Whole-skin samples were obtained backs at 0, 2, 4, 6, age, morphological primary secondary HFs was observed, gene transcript levels insulin-like growth factor-I (IGF-I), epidermal factor (EGF), bone morphogenetic protein 2 (BMP2), transforming β-1, 3 (TGFβ-1, TGFβ-2, TGFβ-3) examined using quantitative real-time polymerase chain reaction (PCR). Additionally, Wnt family member 10b (Wnt10b) β-Catenin by PCR western blot, respectively. Results The results showed significant changes differentiation during their first life. IGF-I, EGF, TGFβ-3 significantly lower age than gradually increased thereafter, while BMP2 TGFβ-1 higher those decreased thereafter. also affected Wnt10b level not. However, β-catenin lowest 4 Conclusion Our data that a series occurred weeks. Many genes, such as BMP2, TGFβ-1, TGFβ-3, β-Catenin, participated this process,
ajas.info参考文章(24)
Terry Magnuson, David W. Threadgill, John P. Sundberg, Laura A. Hansen, Stuart H. Yuspa, Margaret E. Hogan, Andrzej Dlugosz, Natalie Alexander, Genetically null mice reveal a central role for epidermal growth factor receptor in the differentiation of the hair follicle and normal hair development American Journal of Pathology. ,vol. 150, pp. 1959- 1975 ,(1997)
Y.-H. Li, K. Zhang, J.-X. Ye, X.-H. Lian, T. Yang, Wnt10b promotes growth of hair follicles via a canonical Wnt signalling pathway. Clinical and Experimental Dermatology. ,vol. 36, pp. 534- 540 ,(2011) , 10.1111/J.1365-2230.2011.04019.X
Sarah M. Rudman, Michael P. Philpott, Geraldine A. Thomas, Terence Kealey, The Role of IGF-I in Human Skin and its Appendages: Morphogen as Well as Mitogen? Journal of Investigative Dermatology. ,vol. 109, pp. 770- 777 ,(1997) , 10.1111/1523-1747.EP12340934
Julie C Little, Gillian E Westgate, Anita Evans, Stewart P Granger, Cytokine Gene Expression in Intact Anagen Rat Hair Follicles Journal of Investigative Dermatology. ,vol. 103, pp. 715- 720 ,(1994) , 10.1111/1523-1747.EP12398584
H. Galbraith, Fundamental hair follicle biology and fine fibre production in animals. Animal. ,vol. 4, pp. 1490- 1509 ,(2010) , 10.1017/S175173111000025X
Brian Bierie, Harold L. Moses, Tumour microenvironment: TGFβ: the molecular Jekyll and Hyde of cancer Nature Reviews Cancer. ,vol. 6, pp. 506- 520 ,(2006) , 10.1038/NRC1926
M. V. Plikus, R. E. Baker, C.-C. Chen, C. Fare, D. de la Cruz, T. Andl, P. K. Maini, S. E. Millar, R. Widelitz, C.-M. Chuong, Self-Organizing and Stochastic Behaviors During the Regeneration of Hair Stem Cells Science. ,vol. 332, pp. 586- 589 ,(2011) , 10.1126/SCIENCE.1201647
Peter Lönn, Anita Morén, Erna Raja, Markus Dahl, Aristidis Moustakas, Regulating the stability of TGFβ receptors and Smads Cell Research. ,vol. 19, pp. 21- 35 ,(2009) , 10.1038/CR.2008.308
Joerg Huelsken, Regina Vogel, Bettina Erdmann, George Cotsarelis, Walter Birchmeier, β-Catenin Controls Hair Follicle Morphogenesis and Stem Cell Differentiation in the Skin Cell. ,vol. 105, pp. 533- 545 ,(2001) , 10.1016/S0092-8674(01)00336-1
Sara Strömberg, Marcus Gry Björklund, Caroline Asplund, Anna Sköllermo, Anja Persson, Kenneth Wester, Caroline Kampf, Peter Nilsson, Ann-Catrin Andersson, Mathias Uhlen, Juha Kononen, Fredrik Ponten, Anna Asplund, A high-throughput strategy for protein profiling in cell microarrays using automated image analysis. Proteomics. ,vol. 7, pp. 2142- 2150 ,(2007) , 10.1002/PMIC.200700199