Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and γδT Cells

摘要: Cell-based therapies using genetically engineered lymphocytes expressing antigen-specific T cell receptors (TCRs) hold promise for the treatment of several types cancers. Almost all studies this modality have focused on transfer TCR from CD8 cytotoxic (CTLs). The innate to other has not been studied. In current study, and adaptive were transfected with human NKT cell-derived TCRα β chain mRNA (the Vα24 Vβ11 chains). When primary cells subsequently stimulated ligand, α-galactosylceramide (α-GalCer), they secreted IFN-γ in a ligand-specific manner. Furthermore when γδT mRNA, demonstrated enhanced proliferation, production antitumor effects after α-GalCer stimulation as compared parental cells. Importantly, TCR-transfected responded both ligands, rendering them bi-potential lymphocytes. Because are unique universal invariant humans, chains do yield mispaired endogenous α transfection. transfection potential be new approach tumor immunotherapy patients various cancer.