Lyophilized HER2-specific PEGylated immunoliposomes for active siRNA gene silencing.
作者： Jie Gao , Jing Sun , Huimei Li , Wei Liu , Yang Zhang
DOI: 10.1016/J.BIOMATERIALS.2009.11.112
关键词:
摘要: The development of a tumor-specific immunoliposome delivering small interfering RNA (siRNA) represents practical way in cancer gene therapy. In this study, we developed PEGylated 3beta-[N-(N', N'-dimethylaminoethane) carbamoyl] cholesterol (DC-Chol)/dioleoylphosphatidyl ethanolamine (DOPE) immunoliposomes conjugated with the Fab' recombinant humanized anti-HER2 monoclonal antibody (PIL) for siRNA delivery. results demonstrated that lyophilized PIL (LPIL) prepared by lyophilization/rehydration method possessed significantly enhanced HER1 gene, model target, silencing ability compared HER2-overexpressing SK-BR3 cells. Among series LPIL different PEGylation degree, containing 2.5%PEG (2.5%PEG LPIL) showed best activity. Confocal microscope studies could specifically bind to cells and were sequentially internalized into them. Using RhoA as therapeutic entrapping anti-RhoA silence expression inhibit cell invasion conclusion, these finding potential use cancers.
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sci-hub.se 参考文章(36)
Lloyd B. Jeffs, Lorne R. Palmer, Ellen G. Ambegia, Cory Giesbrecht, Shannon Ewanick, Ian MacLachlan, A scalable, extrusion-free method for efficient liposomal encapsulation of plasmid DNA Pharmaceutical Research. ,vol. 22, pp. 362- 372 ,(2005) , 10.1007/S11095-004-1873-Z
Yoshie Maitani, Saki Igarashi, Mamiko Sato, Yoshiyuki Hattori, Cationic liposome (DC-Chol/DOPE=1:2) and a modified ethanol injection method to prepare liposomes, increased gene expression. International Journal of Pharmaceutics. ,vol. 342, pp. 33- 39 ,(2007) , 10.1016/J.IJPHARM.2007.04.035
John Charles Marshall Stewart, Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Analytical Biochemistry. ,vol. 104, pp. 10- 14 ,(1980) , 10.1016/0003-2697(80)90269-9
J.Thomas August, Kam W. Leong, Hai-Quan Mao, Krishnendu Roy, Vu L. Troung-Le, Kevin A. Janes, Kevin Y. Lin, Yan Wang, Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency Journal of Controlled Release. ,vol. 70, pp. 399- 421 ,(2001) , 10.1016/S0168-3659(00)00361-8
Crispin R. Dass, Peter F.M. Choong, Selective gene delivery for cancer therapy using cationic liposomes: in vivo proof of applicability. Journal of Controlled Release. ,vol. 113, pp. 155- 163 ,(2006) , 10.1016/J.JCONREL.2006.04.009
Yoshiyuki Hattori, Ayako Hagiwara, Wuxiao Ding, Yoshie Maitani, NaCl improves siRNA delivery mediated by nanoparticles of hydroxyethylated cationic cholesterol with amido-linker. Bioorganic & Medicinal Chemistry Letters. ,vol. 18, pp. 5228- 5232 ,(2008) , 10.1016/J.BMCL.2008.08.070
Keelung Hong, Weiwen Zheng, Andrew Baker, Demetrios Papahadjopoulos, Stabilization of cationic liposome‐plasmid DNA complexes by polyamines and poly(ethylene glycol)‐phospholipid conjugates for efficient in vivo gene delivery FEBS Letters. ,vol. 400, pp. 233- 237 ,(1997) , 10.1016/S0014-5793(96)01397-X
Arndt A.P. Schmitz, Eve-Ellen Govek, Benjamin Böttner, Linda Van Aelst, Rho GTPases: signaling, migration, and invasion. Experimental Cell Research. ,vol. 261, pp. 1- 12 ,(2000) , 10.1006/EXCR.2000.5049
K. Remaut, B. Lucas, K. Braeckmans, J. Demeester, S.C. De Smedt, Pegylation of liposomes favours the endosomal degradation of the delivered phosphodiester oligonucleotides. Journal of Controlled Release. ,vol. 117, pp. 256- 266 ,(2007) , 10.1016/J.JCONREL.2006.10.029
Ye Zhang, Thomas J Anchordoquy, The role of lipid charge density in the serum stability of cationic lipid/DNA complexes Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 1663, pp. 143- 157 ,(2004) , 10.1016/J.BBAMEM.2004.03.004