Metabolic phenotypes of Saccharomyces cerevisiae mutants with altered trehalose 6-phosphate dynamics.

摘要: In Saccharomyces cerevisiae , synthesis of T6P (trehalose 6-phosphate) is essential for growth on most fermentable carbon sources. the present study, metabolic response to glucose was analysed in mutants with different capacities accumulate T6P. A mutant carrying a deletion synthase encoding gene, TPS1 which had no measurable T6P, exhibited impaired ethanol production, showed diminished plasma membrane H + -ATPase activation, and became rapidly depleted nearly all adenine nucleotides were irreversibly converted into inosine. Deletion AMP deaminase AMD1 tps1 strain prevented inosine formation, but did not rescue energy balance or glucose. Neither 90%-reduced content observed expressing Tps1 protein from Yarrowia lipolytica nor hyperaccumulation tps2 significant effects fermentation rates, sources activation. However, intracellular metabolite dynamics pH homoeostasis strongly affected by changes concentrations. Hyperaccumulation caused an increase cytosolic reduced rates non-fermentable sources, emphasizing crucial role trehalose pathway regulation respiratory fermentative metabolism.