Investigating the mechanisms of glyphosate resistance in Lolium multiflorum

摘要: Evolved resistance to the herbicide glyphosate has been reported in eleven weed species, including Lolium multiflorum. Two glyphosate-resistant L. multiflorum populations were collected, one from Chile (SF) and Oregon, USA (OR), mechanisms conferring studied. Based on a Petri dish dose–response bioassay, OR SF two fivefold more resistant when compared susceptible (S) population, respectively; however, based whole-plant both than S implying that different might be involved. The population accumulated three times shikimic acid leaf tissue 96 h after application populations, respectively. There no differences between 14C-glyphosate uptake; patterns of translocation significantly different. In greater percentage absorbed by plant moved distal treated section tip leaf. contrast, non-treated leaves stem. cDNA sequence analysis EPSP synthase gene indicated proline 106 serine amino substitution. Here, we report is conferred mechanisms, limited (nontarget site-based) mutation (target site-based).