Anti-inflammative effect of glycyrrhizin on rat thermal injury via inhibition of high-mobility group box 1 protein.

摘要: Abstract Aim Glycyrrhizin (Gly) has been reported as an inhibitor of extracellular HMGB1 (high-mobility group box 1 protein) cytokine's activity, and protects spinal cord, liver, heart brain against ischemia-reperfusion-induced injury in rats. The purpose this study was to investigate the protective effect Gly rat skin thermal model elucidate underlying mechanisms. Methods Twenty-four male Sprague-Dawley rats (200–250g) were randomly divided into control group, vehicle-treated Gly-treated burn groups, each contained eight animals. In latter two subjected 30% TBSA (Total Body Surface Area) full-thickness scald injury. glycyrrhizin (60mg/kg) administered intraperitoneally immediately after at 24th hour burn; Ringer's solution (4ml/kg, a vehicle) burn. animals sacrificed 48h Aortic blood samples obtained detect tumor necrosis factor-α (TNF-α) interleukin-1β (IL-1β) with ELISA (Enzyme-Linked Immuno Sorbent Assay) kits. Lung, liver kidney tissue collected determine expression mRNA protein. level semiquantitatively measured by Real-Time PCR using β-actin internal standard, protein HMGBI determined Western blot. Results Severe caused significant increase plasma TNF-α IL-1β versus ( P Conclusion These results demonstrate that possesses anti-inflammation protect remote organs from burn-induced