Overproduction and secretion of Bacillus circulans endo-beta-1,3-1,4-glucanase gene (bglBC1) in B. subtilis and B. megaterium.
作者： Ji-Yeon Kim
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摘要: A gene coding for endo-beta-1,3-1,4-glucanase (lichenase) containing a recombinant plasmid, pLL200K, was transferred from Bacillus circulans into new shuttle pLLS920, by ligating linearized DNAs of pLL200K and pUB110. B. subtilis RM125 megaterium ATCC14945 transformed with pLLS920 produced the endo-beta-1,3-1,4-glucanase. The enzyme during active growth maximum activity. (pLLS920) 83 times (8522 mU ml(-1)) more than that donor cells (103 ml(-1)). 7 (735 cells. While E. coli secreted only about 10% enzyme, excreted completely medium 98%. plasmid stable in (98%), (51%) but not (29%).
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