Quantitative imaging of platinum based on laser ablation-inductively coupled plasma-mass spectrometry to investigate toxic side effects of cisplatin.

摘要: This work presents a quantitative bioimaging method for platinum based on laser ablation-inductively coupled plasma-mass spectrometry and its application biomedical study concerning toxic side effects of cisplatin. To trace the histopathology back to cisplatin, was localized quantified in major functional units testicle, cochlea, kidney, nerve brain sections from cisplatin treated mice. The direct consideration histology enables precise interpretation Pt images novel evaluation approach allows significantly more investigations than pure image. For first time, detected all injured structures including organ Corti cochlea seminiferous tubule testicle. In this way, proximal Leydig cells stria vascularis fibers sciatic nerves are confirmed as targets these organs. However, accumulation almost investigated also raises questions about complex pathogenesis indirect interruption several biological processes.