Spc110p: assembly properties and role in the connection of nuclear microtubules to the yeast spindle pole body.

摘要: Spc110p is an essential component of the budding yeast spindle pole body (SPB). It binds calmodulin and contains a long central coiled-coil rod which acts as spacer element between plaque SPB ends nuclear or microtubules. This suggests that function to connect microtubules SPB. To confirm this, we examined phenotype ts alleles SPC110, one mutation in binding site was suppressed by overexpression calmodulin. The fail form functional mitotic because are not properly connected We also toxic either wild-type truncated version containing deletion most domain. Both these proteins large ordered spheroidal polymers nucleus. polymerization appears be initiated inside from position where normally located, polymer grows size it severs connection were purified composed A model for structure proposed.