Development of T cell receptor-gamma delta cells. Phenotypic and functional correlations of T cell receptor-gamma delta thymocyte maturation.

摘要: The development of TCR-gamma delta cells during thymic ontogeny has been studied using fetal organ cultures normal and transgenic (Tg) mice. expression the cell-surface markers--heat stable Ag (HSA), MEL-14, CD5, CD25 (IL-2R), CD44 (Pgp-1)--correlated with maturation. As thymus developed, there was an increase in HSA-, CD5dull, CD44+ for each cell subset. Moreover, recombination activating genes-1 -2 (RAG-1 RAG-2) also correlated maturation as only HSA+ transcribed these genes. Cyclosporin A inhibited thymocytes if it introduced early by arresting differentiation at stage. Immature isolated from both Tg mice did not respond to nominal or anti-TCR mAb unless exogenous IL-2 added cultures. In contrast, HSA- responded TCR/ligand interactions absence additional IL-2. Finally, functionally mature could be induced vitro. Interaction Tg+ anti-TCR-gamma Ag-bearing stromal resulted RAG-1 RAG-2 down-regulation. These data strongly suggest that HSA+, RAG+ differentiate into a more stage under pressure positive selection is regulated manner similar TCR-alpha beta cells. addition, ability inhibit combined findings can induce suggests depends on receptor-mediated physiologic stimuli delivered development.